Assay guide

Assay Guidance Manual

This Assay Guidance Manual by Eli Lilly & Company and the National Center for Advancing Translational Sciences (NCATS),  is considered as "tribe knowledge" on assay development, assay technologies and high throughput screening for investigators interested in developing assays for probe/drug discovery and development.

This manual will be continuously updated with contributions from experienced scientists. A previous version is provided here for your information only.

High Throughput Screening for 3D Cell Culture Models

A basic intro on Youtube


Automating and Miniaturizing Gene Expression Assays via High-Throughput RT-qPCR

Luciferase reporter assay vs. RT-qPCR assay in HTS format. Check GEN report on Sep 15, 2014